Which of the following indicates a negative mono spot test?

A negative mono spot test is indicated by no agglutination. This test is designed to detect heterophile antibodies, which are typically present in the case of infectious mononucleosis, commonly associated with Epstein-Barr virus infection. When the test is performed, the presence of heterophile antibodies would cause the red blood cells to agglutinate, or clump together, resulting in a positive test. Therefore, if there is no agglutination observed, it signifies that the antibodies are either not present or not present in sufficient quantities, leading to a negative result.

Clear agglutination, severe clumping, and pale yellow color do not correlate with a negative test. Clear agglutination would suggest the presence of antibodies, while severe clumping indicates a strong positive reaction. A pale yellow color is not a standard indicator in the reading of a mono spot test and is typically unrelated to the results of agglutination.